monoclonal antibodies against tgf β  (Bioss)

Journal: International Journal of Cardiology. Heart & Vasculature

Article Title: Atrial fibrillation in human patients is associated with increased collagen type V and TGFbeta1

doi: 10.1016/j.ijcha.2023.101327

Figure Lengend Snippet: Confocal images of TGF-ß1 (red) and collagen V (green) in SR, pAF and cAF groups. Cardiomyocytes are stained blue with Alexa633-phalloidin and nuclei are stained white with DAPI. Note that TGFß1 is mainly confined to interstitial cells and, compared with SR patients, is obviously increased in the pAF and cAF groups. Note also that increases in TGF-ß1 are associated with increased abundance of collagen V. The lower left and right graphs are quantifications of TGFß1 expressed as arbitrary units of fluorescence per 1 mm 2 surface area or the number of TGF-β1-positive cells per 1 mm 2 of atrial tissue area.

Article Snippet: A monoclonal antibody against vimentin (clone V-9, Sigma) was directly conjugated with Cy3 (clone V9, Sigma) and a monoclonal antibody against TGF-β1 (MAB240, R&D) was used in dilutions as previously described .

Techniques: Staining, Fluorescence

Journal: Cell Communication and Signaling : CCS

Article Title: Connexin43 mediates NF-κB signalling activation induced by high glucose in GMCs: involvement of c-Src

doi: 10.1186/1478-811X-11-38

Figure Lengend Snippet: Regulation of high glucose-induced NF-κB nuclear translocation and c-Src activity by Cx43 is independent of GJIC. GMCs were transfected with GFP-Cx43 or Flag-Cx43CT under the condition of normal glucose (NG; 5.5 mmol/L). After 48 h of transfection, GMCs were exposed to high glucose (HG; 30mmol/L) for 30 min. ( A ) Photomicrographs obtained after Lucifer yellow scrape-loading in GMCs transfected with GFP-Cx43 and Flag-Cx43CT (magnification 100×, upper panel). Phase contrast views are also provided (lower panel). Scale bar represents 100 μm. ( B ) Proteins were extracted for analysis of NF-κB p65 nuclear translocation by immunoblotting. Histone H1.4 and α-tubulin were measured by immunoblotting as a loading control. ( C ) c-Src was immunoprecipitated with an anti-c-Src antibody and Flag, phosphorylation of Tyr416 on c-Src (Y416-c-Src) and total c-Src were analyzed by immunoblotting. Experiments were performed at least three times with similar results. * P <0.05 vs. normal glucose-treated group, # P <0.05 vs. 30 mmol/L glucose-treated group.

Article Snippet: The antibodies included mouse monoclonal antibodies against connexin43, NF-κB p65, Inhibitor of κB-α (IκB-α), p-Tyr and FN, rabbit polyclonal antibody against c-Src, goat polyclonal anti-body against ICAM-1 and ZO-1 (Santa Cruz Biotechnology, Santa Cruz, CA), rabbit monoclonal antibodies against phospho-c-Src (Tyr), connexin43, phospho-IκB-α (Ser) and TGF-β (Cell Signalling, Danvers, MA), rabbit monoclonal antibodies against Histone H1.4 and α-tubulin (Sigma–Aldrich, St. Louis, MO), mouse monoclonal antibodies against Thy-1.1 and RECA-1 (Abcam, Cambridge, MA).

Techniques: Translocation Assay, Activity Assay, Transfection, Western Blot, Immunoprecipitation

Journal: Antioxidants

Article Title: Impact of Dietary Lavender Essential Oil on the Growth and Fatty Acid Profile of Breast Muscles, Antioxidant Activity, and Inflammatory Responses in Broiler Chickens

doi: 10.3390/antiox11091798

Figure Lengend Snippet: Immunostained positive TGF-β cells (red arrows) in the liver of different experimental chicken groups. ( E ): 0.08, ( F ): 0.45, ( G ): 0.93, and ( H ): 7.2% for LEO0, LEO200, LEO400, and LEO600 groups.

Article Snippet: Then, one to two drops of the supersensitive primary monoclonal antibody against TGF-β (Cat. BAF240, Novus Biologicals, Briarwood Avenue, Centennial, CO, USA) were added to these sections; then, the slides were counterstained using hematoxylin and visualized under the microscope.

Techniques:

Journal: Antioxidants

Article Title: Impact of Dietary Lavender Essential Oil on the Growth and Fatty Acid Profile of Breast Muscles, Antioxidant Activity, and Inflammatory Responses in Broiler Chickens

doi: 10.3390/antiox11091798

Figure Lengend Snippet: The morphometric analytic data in the spleen and liver of different experimental groups. LEO0, LEO200, LEO400, and LEO600: basal diets supplemented with 0, 200, 400, and 600 mg Kg − 1 lavender essential oil, respectively. a, b, c, d Means carrying different superscripts are significantly different at ( p < 0.05). TGF-β: transforming growth factor beta.

Article Snippet: Then, one to two drops of the supersensitive primary monoclonal antibody against TGF-β (Cat. BAF240, Novus Biologicals, Briarwood Avenue, Centennial, CO, USA) were added to these sections; then, the slides were counterstained using hematoxylin and visualized under the microscope.

Techniques:

Journal: Antioxidants

Article Title: Impact of Dietary Lavender Essential Oil on the Growth and Fatty Acid Profile of Breast Muscles, Antioxidant Activity, and Inflammatory Responses in Broiler Chickens

doi: 10.3390/antiox11091798

Figure Lengend Snippet: Immunostained positive TGF-β cells (red arrows) in the spleen of different experimental chicken groups ( E ): 0.21, ( F ): 0.82, ( G ): 1.06, and ( H ): 2.8% for LEO0, LEO200, LEO400, and LEO600 groups, respectively.

Article Snippet: Then, one to two drops of the supersensitive primary monoclonal antibody against TGF-β (Cat. BAF240, Novus Biologicals, Briarwood Avenue, Centennial, CO, USA) were added to these sections; then, the slides were counterstained using hematoxylin and visualized under the microscope.

Techniques: